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A huge flock of Christmas turkeys is set to be culled after a H5N8 strain of bird flu took over the farm.
At least 30,000 birds are expected to be culled following the discovery in Norfolk, which comes just a month after 10,500 turkeys were killed in North Yorkshire.
The farm has not been named, but the Department for Environment Food and Rural Affairs have published a map pin-pointing its location.
Biosecurity measures have been put in place within the 3km surrounding the farm as a "precaution".
Security staff were pictured wearing high visibility jackets outside a private entrance, leading to what appears to be a polytunnel-style turkey shelter.
A DEFRA spokesperson confirmed that 30,000 turkeys on the Norfolk farm were in the process of being humanly culled as a precaution.
The spokesman said: "A veterinary investigation is on-going on this site to identify the likely source of infection and establish how long the disease may have been present on the infected premises."
It also said the strain appears "closely related" to the virus "currently circulating in wild and captive birds in Europe."
DEFRA said: "The strain of HPAI H5N8 which has been confirmed in several poultry premises in England appears closely related to the virus currently circulating in wild and captive birds in Europe.
"Bird keepers should remain alert for any signs of disease, report suspected disease immediately and ensure they are maintaining good biosecurity on their premises."
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The government body also said the turkeys would be killed as "humanely as possible" and said it was introducing tough new measures to combat the disease.
On Friday December 4, DEFRA said all poultry flocks including free range birds will have to be kept indoors in England, Scotland and Wales from December 14.
The move will help to keep them separate from potentially infectious wild birds, DEFRA said, and follows guidance introduced in the Netherlands.
Poultry keepers are being urged to prepare for the new measures by erecting additional housing or self-contained netted areas.
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