The pair were exposed to the deadly nerve agent in Amesbury, Wiltshire, at the end of last month, with Dawn dying eight days later.
Salisbury District Hospital Director of Nursing Lorna Wilkinson said: "I am pleased to confirm that earlier today, Charlie Rowley was discharged from hospital.
"Charlie has been through an appalling experience most of us could never imagine.
"Today is a very welcome milestone in his recovery and all of us here at Salisbury Hospital wish him well as he continues to get better.
"The progress he has made is a testament to the remarkable clinical team who have worked tirelessly, supported by brilliant behind the scenes staff.
It is believed Dawn, 44 was exposed to at least 10 times the amount of nerve agent used on ex-KGB colnel Sergei Skripal and his daughter Yulia.
Cops are probing a theory the poison was in a discarded perfume bottle.
The pair are not known spies or assassins but are believed to be part of Putin's feared GRU military intelligence service, US media reports.
Investigators combing through hours of CCTV footage discovered the "fresh identities" by cross-checking them with passenger lists of the commercial flight used to flee Britain.
A source told CNN facial recognition technology was used to identify the suspects, who were said to be travelling on fake names.
The media outlet claimed the pair's departure was discussed in a coded Russian message to Moscow – intercepted by a British base in Cyprus.
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